Laboratory of Adaptive Immunity|Institute of Molecular Genetics

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Publications

2023

  • Freke GM, Martins T, Davies RJ, Beyer T, Seda M, Peskett E, Haq N, Prasai A, Otto G, Jeyabalan Srikaran J, Hernandez V, Diwan GD, Russell RB, Ueffing M, Huranova M, Boldt K, Beales PL, Jenkins D: De-Suppression of Mesenchymal Cell Identities and Variable Phenotypic Outcomes Associated with Knockout of . Cells 2023 12(22). [pubmed] [doi]
  • Horkova V, Drobek A, Paprckova D, Niederlova V, Prasai A, Uleri V, Glatzova D, Kraller M, Cesnekova M, Janusova S, Salyova E, Tsyklauri O, Kadlecek TA, Krizova K, Platzer R, Schober K, Busch DH, Weiss A, Huppa JB, Stepanek O: Unique roles of co-receptor-bound LCK in helper and cytotoxic T cells. Nat Immunol 2023 24(1): 174-185. [pubmed] [doi]
  • Janusova S, Krchlikova V, Hron T, Elleder D, Stepanek O: Identification of GC-rich LAT genes in birds. PLoS One 2023 18(4): e0283431. [pubmed] [doi]
  • Niederlova V, Tsyklauri O, Kovar M, Stepanek O: IL-2-driven CD8 T cell phenotypes: implications for immunotherapy. Trends Immunol 2023 44(11): 890-901. [pubmed] [doi]
  • Paprckova D, Salyova E, Michalik J, Stepanek O: Bystander activation in memory and antigen-inexperienced memory-like CD8 T cells. Curr Opin Immunol 2023 82: 102299. [pubmed] [doi]
  • Tsyklauri O, Chadimova T, Niederlova V, Kovarova J, Michalik J, Malatova I, Janusova S, Ivashchenko O, Rossez H, Drobek A, Vecerova H, Galati V, Kovar M, Stepanek O: Regulatory T cells suppress the formation of potent KLRK1 and IL-7R expressing effector CD8 T cells by limiting IL-2. Elife 2023 12. [pubmed] [doi]
  • Vancikova S, Benešová I, Černý V, Lázničková P, Miková E, Šmídová B, Věcek J, Zuani M, Niederlova V: Czech Young Immunologists: Bound by science, united in purpose. Eur J Immunol 2023, e2350737. [pubmed] [doi]

2022

  • Binó L, Mikulenková E, Štepánek L, Bernatík O, Vysloužil D, Pejšková P, Gorilák P, Huranová M, Varga V, Čajánek L: A protocol for generation and live-cell imaging analysis of primary cilia reporter cell lines. STAR Protoc 2022 3(1): 101199. [pubmed] [doi]
  • Knizkova D, Pribikova M, Draberova H, Semberova T, Trivic T, Synackova A, Ujevic A, Stefanovic J, Drobek A, Huranova M, Niederlova V, Tsyklauri O, Neuwirth A, Tureckova J, Stepanek O, Draber P: CMTM4 is a subunit of the IL-17 receptor and mediates autoimmune pathology. Nat Immunol 2022. [pubmed] [doi]
  • Niederlova V, Kazer SW, Ordovas-Montanes J: Are we there yet? An immune field trip through human embryonic development. Immunity 2022 55(7): 1153-1155. [pubmed] [doi]
  • Paprckova D, Niederlova V, Moudra A, Drobek A, Pribikova M, Janusova S, Schober K, Neuwirth A, Michalik J, Huranova M, Horkova V, Cesnekova M, Simova M, Prochazka J, Balounova J, Busch DH, Sedlacek R, Schwarzer M, Stepanek O: Self-reactivity of CD8 T-cell clones determines their differentiation status rather than their responsiveness in infections. Front Immunol 2022 13: 1009198. [pubmed] [doi]

2021

  • Cassioli C, Onnis A, Finetti F, Capitani N, Brunetti J, Compeer EB, Niederlova V, Stepanek O, Dustin ML, Baldari CT: The Bardet-Biedl syndrome complex component BBS1 controls T cell polarity during immune synapse assembly. J Cell Sci 2021 134(16). [pubmed] [doi]
  • Gorilak P, Pružincová M, Vachova H, Olšinová M, Schmidt Cernohorska M, Varga V: Expansion microscopy facilitates quantitative super-resolution studies of cytoskeletal structures in kinetoplastid parasites. Open Biol 2021 11(9): 210131. [pubmed] [doi]
  • Horkova V, Stepanek O: A LoCK at the T cell dock. Science 2021 372(6546): 1038-1039. [pubmed] [doi]
  • Karimi M, Guo Y, Pallikonda HA, Horková V, Wang YF, Gil SR, Rodriguez-Esteban G, Robles-Rebollo I, Bruno L, Georgieva R, Patel B, Elliptt J, Dore MH, Dauphars D, Krangel MS, Lenhard B, Heyn H, Fisher AG, Štěpánek O, Merkenschlager M: The order and logic of CD4 versus CD8 lineage choice and differentiation in mouse thymus. Nature Communications 2021 12(99). [pubmed] [doi]
  • Kubalová I., Schmidt Černohorská M., Huranová M., Weisshart K., Houben A., Schubert V.: Chapter 10 – A protocol to expand plant nuclei Methods in Cell Biology 2021 161: 197-216. [pubmed] [doi]
  • Moudra A, Niederlova V, Novotny J, Schmiedova L, Kubovciak J, Matejkova T, Drobek A, Pribikova M, Stopkova R, Cizkova D, Neuwirth A, Michalik J, Krizova K, Hudcovic T, Kolar M, Kozakova H, Kreisinger J, Stopka P, Stepanek O: Phenotypic and Clonal Stability of Antigen-Inexperienced Memory-like T Cells across the Genetic Background, Hygienic Status, and Aging. J Immunol 2021 206(9): 2109-2121. [pubmed] [doi]
  • Niederlova V, Tsyklauri O, Chadimova T, Stepanek O: CD8 Tregs revisited: A heterogeneous population with different phenotypes and properties. Eur J Immunol 2021 51(3): 512-530. [pubmed] [doi]
  • Paprckova D, Stepanek O: Narcissistic T cells: reactivity to self makes a difference. FEBS J 2021 288(6): 1778-1788. [pubmed] [doi]
  • Ruppova K, Lim JH, Fodelianaki G, August A, Neuwirth A: Eosinophils are dispensable for development of MOG-induced experimental autoimmune encephalomyelitis in mice. Immunol Lett 2021 239: 72-76. [pubmed] [doi]
  • Tsyklauri O, Niederlova V, Forsythe E, Prasai A, Drobek A, Kasparek P, Sparks K, Trachtulec Z, Prochazka J, Sedlacek R, Beales P, Huranova M, Stepanek O: Bardet-Biedl Syndrome ciliopathy is linked to altered hematopoiesis and dysregulated self-tolerance. EMBO Rep 2021, e50785. [pubmed] [doi]

2020

  • Dobbelaere J, Schmidt Cernohorska M, Huranova M, Slade D, Dammermann A: Cep97 Is Required for Centriole Structural Integrity and Cilia Formation in Drosophila. Curr Biol 2020. [pubmed] [doi]
  • Draberova H, Janusova S, Knizkova D, Semberova T, Pribikova M, Ujevic A, Harant K, Knapkova S, Hrdinka M, Fanfani V, Stracquadanio G, Drobek A, Ruppova K, Stepanek O, Draber P: Systematic analysis of the IL-17 receptor signalosome reveals a robust regulatory feedback loop. EMBO J 2020, e104202. [pubmed] [doi]
  • Horkova V, Drobek A, Mueller D, Gubser C, Niederlova V, Wyss L, King CG, Zehn D, Stepanek O: Dynamics of the Coreceptor-LCK Interactions during T Cell Development Shape the Self-Reactivity of Peripheral CD4 and CD8 T Cells. Cell Rep 2020 30(5): 1504-1514.e7. [pubmed] [doi]
  • Kralova J, Drobek A, Prochazka J, Spoutil F, Fabisik M, Glatzova D, Borna S, Pokorna J, Skopcova T, Angelisova P, Gregor M, Kovarik P, Sedlacek R, Brdicka T: Dysregulated NADPH Oxidase Promotes Bone Damage in Murine Model of Autoinflammatory Osteomyelitis. J Immunol 2020 204(6): 1607-1620. [pubmed] [doi]
  • Kubalová I, Schmidt Černohorská M, Huranová M, Weisshart K, Houben A, Schubert V: Prospects and limitations of expansion microscopy in chromatin ultrastructure determination. Chromosome Res 2020 28(3): 355-368. [pubmed] [doi]
  • Paprckova D, Stepanek O: Narcissistic T cells: reactivity to self makes a difference. FEBS J 2020. [pubmed] [doi]
  • Prasai A, Schmidt Cernohorska M, Ruppova K, Niederlova V, Andelova M, Draber P, Stepanek O, Huranova M: The BBSome assembly is spatially controlled by BBS1 and BBS4 in human cells. J Biol Chem 2020. [pubmed] [doi]
  • Vobořil M, Brabec T, Dobeš J, Šplíchalová I, Březina J, Čepková A, Dobešová M, Aidarova A, Kubovčiak J, Tsyklauri O, Štěpánek O, Beneš V, Sedláček R, Klein L, Kolář M, Filipp D: Toll-like receptor signaling in thymic epithelium controls monocyte-derived dendritic cell recruitment and Treg generation. Nat Commun 2020 11(1): 2361. [pubmed] [doi]

2019

  • Arakel EC, Huranova M, Estrada AF, Rau EM, Spang A, Schwappach B: Dissection of GTPase-activating proteins reveals functional asymmetry in the COPI coat of budding yeast. J Cell Sci 2019 132(16). [pubmed] [doi]
  • Courtney AH, Shvets AA, Lu W, Griffante G, Mollenauer M, Horkova V, Lo WL, Yu S, Stepanek O, Chakraborty AK, Weiss A: CD45 functions as a signaling gatekeeper in T cells. Sci Signal 2019 12(604). [pubmed] [doi]
  • Lo WL, Shah NH, Rubin SA, Zhang W, Horkova V, Fallahee IR, Stepanek O, Zon LI, Kuriyan J, Weiss A: Slow phosphorylation of a tyrosine residue in LAT optimizes T cell ligand discrimination. Nat Immunol 2019. [pubmed] [doi]
  • Niederlova V, Modrak M, Tsyklauri O, Huranova M, Stepanek O: Meta-analysis of genotype-phenotype associations in Bardet-Biedl syndrome uncovers differences among causative genes. Hum Mutat 2019. [pubmed] [doi]

2018

  • Drobek A, Moudra A, Mueller D, Huranova M, Horkova V, Pribikova M, Ivanek R, Oberle S, Zehn D, McCoy KD, Draber P, Stepanek O: Strong homeostatic TCR signals induce formation of self-tolerant virtual memory CD8 T cells. EMBO J 2018 37(14). [pubmed] [doi]
  • Lafont E, Draber P, Rieser E, Reichert M, Kupka S, de Miguel D, Draberova H, von Mässenhausen A, Bhamra A, Henderson S, Wojdyla K, Chalk A, Surinova S, Linkermann A, Walczak H: TBK1 and IKKε prevent TNF-induced cell death by RIPK1 phosphorylation. Nat Cell Biol 2018 20(12): 1389-1399.
    [pubmed] [doi]
  • Lo WL, Shah NH, Ahsan N, Horkova V, Stepanek O, Salomon AR, Kuriyan J, Weiss A: Lck promotes Zap70-dependent LAT phosphorylation by bridging Zap70 to LAT. Nat Immunol 2018. [pubmed] [doi]
  • Peltzer N, Darding M, Montinaro A, Draber P, Draberova H, Kupka S, Rieser E, Fisher A, Hutchinson C, Taraborrelli L, Hartwig T, Lafont E, Haas TL, Shimizu Y, Böiers C, Sarr A, Rickard J, Alvarez-Diaz S, Ashworth MT, Beal A, Enver T, Bertin J, Kaiser W, Strasser A, Silke J, Bouillet P, Walczak H: LUBAC is essential for embryogenesis by preventing cell death and enabling haematopoiesis. Nature 2018 557(7703): 112-117. [pubmed] [doi]
  • Michaela Pribikova, Alena Moudra, Ondrej Stepanek: Opinion: Virtual memory CD8 T cells and lymphopenia-induced memory CD8 T cells represent a single subset: Homeostatic memory T cells. Immunology Letters 2018 203: 57-61.[pubmed] [doi] [PDF]

2017

  • Bosch AJT, Bolinger B, Keck S, Stepanek O, Ozga AJ, Galati-Fournier V, Stein JV, Palmer E: A minimum number of autoimmune T cells to induce autoimmunity? Cell Immunol 2017 316: 21-31. [pubmed] [doi]

2016

  • Hrdinka M, Sudan K, Just S, Drobek A, Stepanek O, Schlüter D, Reinhold D, Jordan BA, Gintschel P, Schraven B, Kreutz MR: Normal Development and Function of T Cells in Proline Rich 7 (Prr7) Deficient Mice. PLoS One 2016 11(9): e0162863. [pubmed] [doi]
  • Huranová M, Štěpánek O: Role of actin cytoskeleton at multiple levels of T cell activation AIMS Molecular Science 2016 3(4): 585-596. [doi]
  • Palmer E, Drobek A, Stepanek O: Opposing effects of actin signaling and LFA-1 on establishing the affinity threshold for inducing effector T-cell responses in mice. Eur J Immunol 2016 46(8): 1887-901. [pubmed] [doi]

2014

  • Stepanek O, Draber P, Horejsi V: Palmitoylated transmembrane adaptor proteins in leukocyte signaling. Cell Signal 2014 26(5): 895-902. [pubmed] [doi]
  • Stepanek O, Prabhakar AS, Osswald C, King CG, Bulek A, Naeher D, Beaufils-Hugot M, Abanto ML, Galati V, Hausmann B, Lang R, Cole DK, Huseby ES, Sewell AK, Chakraborty AK, Palmer E: Coreceptor scanning by the T cell receptor provides a mechanism for T cell tolerance. Cell 2014 159(2): 333-45. [pubmed] [doi]

2013

  • Stepanek O, Draber P, Drobek A, Horejsi V, Brdicka T: Nonredundant roles of SRC-family kinases and syk in the initiation of B-cell antigen receptor signaling. J Immunol 2013 190(4): 1807-18. [pubmed] [doi]

2012

  • Draber P, Stepanek O, Hrdinka M, Drobek A, Chmatal L, Mala L, Ormsby T, Angelisova P, Horejsi V, Brdicka T: LST1/A is a myeloid leukocyte-specific transmembrane adaptor protein recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to the plasma membrane. J Biol Chem 2012 287(27): 22812-21. [pubmed] [doi]

2011

  • Draber P, Vonkova I, Stepanek O, Hrdinka M, Kucova M, Skopcova T, Otahal P, Angelisova P, Horejsi V, Yeung M, Weiss A, Brdicka T: SCIMP, a transmembrane adaptor protein involved in major histocompatibility complex class II signaling. Mol Cell Biol 2011 31(22): 4550-62. [pubmed] [doi]
  • Hrdinka M, Dráber P, Stepánek O, Ormsby T, Otáhal P, Angelisová P, Brdicka T, Paces J, Horejsí V, Drbal K: PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis. J Biol Chem 2011 286(22): 19617-29. [pubmed] [doi]
  • Stepanek O, Brdicka T, Angelisova P, Horvath O, Spicka J, Stockbauer P, Man P, Horejsi V: Interaction of late apoptotic and necrotic cells with vitronectin. PLoS One 2011 6(5): e19243. [pubmed] [doi]
  • Stepanek O, Kalina T, Draber P, Skopcova T, Svojgr K, Angelisova P, Horejsi V, Weiss A, Brdicka T: Regulation of Src family kinases involved in T cell receptor signaling by protein-tyrosine phosphatase CD148. J Biol Chem 2011 286(25): 22101-12. [pubmed] [doi]